SBB09 Construct40: Difference between revisions
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Jong H. Shin (talk | contribs) No edit summary |
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<pre> | <pre> | ||
Construction of | Construction of upaG (long) basic part | ||
PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) | PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) | ||
Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L) | Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L) |
Revision as of 00:28, 10 February 2009
Construction of upaG (long) basic part PCR js001F/js002R on Escherichia coli CFT073 (4228 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, 2974+910, L) Product is Him_L1_L2_beta {<Him_L1_L2_beta!} ---------------------------------------- js001F CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg js002R CTGATggatccCCACtgaataccggcaccga