SBB09 Construct39
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Construction of <a~Inv-long> basic part Bcr0002 PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-Bcr0002 {a~Inv-long>} ----- Ocr0001-F forward oligo for biobricking of {a~Inv-long>} ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg Ocr0002-R reverse oligo for biobricking of {a~Inv-long>} ccaaaGGATCCctgctggcctcAagactggatacc
JCA: Use Douglas's Odmd002R for your reverse; you need to use the M numbers for naming
Construction of <a~Inv-long> basic part Bcr0002 PCR Ocr0001-F/Odmd002R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-M10031 {a~Inv-long>} ----- Ocr0001-F forward oligo for biobricking of {a~Inv-long>} ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg Odmd002R Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg