SBB09 Construct39: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Construction of <a~Inv-long> basic part PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3040+909, L) Product is pBca9495CA-Bcr0...) |
No edit summary |
||
| Line 1: | Line 1: | ||
<pre> | |||
Construction of <a~Inv-long> basic part | Construction of <a~Inv-long> basic part | ||
| Line 9: | Line 10: | ||
Ocr0002-R reverse oligo for biobricking of {a~Inv-long>} | Ocr0002-R reverse oligo for biobricking of {a~Inv-long>} | ||
ccaaaGGATCCctgctggcctcAagactggatacc | ccaaaGGATCCctgctggcctcAagactggatacc | ||
</pre> | |||
Revision as of 18:40, 9 February 2009
Construction of <a~Inv-long> basic part
PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3040+909, L)
Product is pBca9495CA-Bcr0002 {a~Inv-long>}
-----
Ocr0001-F forward oligo for biobricking of {a~Inv-long>}
ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg
Ocr0002-R reverse oligo for biobricking of {a~Inv-long>}
ccaaaGGATCCctgctggcctcAagactggatacc
