SBB09 Construct39: Difference between revisions
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(New page: Construction of <a~Inv-long> basic part PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3040+909, L) Product is pBca9495CA-Bcr0...) |
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Revision as of 18:35, 9 February 2009
Construction of <a~Inv-long> basic part
PCR Ocr0001-F/Ocr0002-R on pAC-TetInvdBg (2749 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3040+909, L) Product is pBca9495CA-Bcr0002 {a~Inv-long>}
Ocr0001-F forward oligo for biobricking of {a~Inv-long>} ccaaaGAATTCatgAGATCTaattatgatggttttccagccaatcagtg Ocr0002-R reverse oligo for biobricking of {a~Inv-long>} ccaaaGGATCCctgctggcctcAagactggatacc