SBB09 Construct1

From OpenWetWare
Revision as of 19:26, 18 January 2009 by JCAnderson (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search 
PCR ca998/ca1034 on pSB1A2-Bca9033     (~350 bp, EcoRI/BamHI)
Sub into pBca9145-Bca1089              (EcoRI/BamHI, Large)
Product is pBca1020-Bca1034    {fdhF2}
----
ca1034 Reverse biobrick oligo for fdhF
CACTTggatccAATAGGGGCAAACCGTGACGAC



PCR ca9394/ca9395 on colE2      (1758 bp, BglII/XhoI)
Sub into pBca9145-Bca1089           (BglII/XhoI, 2057+696, L)
Product is pBca9145-Bca9394      {ceaB}
----
ca9394   Cloning of ceaB   ccaaaAGATCTatgagcggtggcgatggacg
ca9395   Cloning of ceaB   gctagCTCGAGttaGGATCCtcaatatgtcgcttaggtgt
Retrieved from "https://openwetware.org/mediawiki/index.php?title=SBB09_Construct1&oldid=278227"