SBB09Ntbk-TimHsiau: Difference between revisions
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==[[User:Tim Hsiau|Tim Hsiau]] 19:33, 10 February 2009 (EST)== | ==[[User:Tim Hsiau|Tim Hsiau]] 19:33, 10 February 2009 (EST)== | ||
Grading project balls output! | Grading project balls output! | ||
=== === | ==[[User:Tim Hsiau|Tim Hsiau]] 23:06, 11 February 2009 (EST)== | ||
Found site to do genomic in silico PCR http://insilico.ehu.es/PCR/ | |||
Prepro prediction http://www.cbs.dtu.dk/services/SignalP/ | |||
==[[User:Tim Hsiau|Tim Hsiau]] 18:24, 24 March 2009 (EDT)== | |||
--Use 20 tips on MP20! | |||
Diluted 3 microliter in 50 microliter water. | |||
--want to do transformations | |||
--plating on LB agar -> transposed | |||
==[[User:Tim Hsiau|Tim Hsiau]] 14:23, 25 March 2009 (EDT)== | |||
Having a layout conducive to multichannel pipetting is much more important than "saving wells" | |||
Need to put > 1.5 mL of LB agar into blocks, otherwise the agar shrinks | |||
The robot shouldn't dip into the well so much when pipetting antiobiotics. Currently, it is touching the walls of the well and might be picking up other antibiotics and cross contaminating the stock solutions | |||
==[[User:Tim Hsiau|Tim Hsiau]] 13:57, 26 March 2009 (EDT)== | |||
Plated 20µL of culture, this amount seems to work quite well on the 24 well agar LB plates | |||
Some righty liquid cultures didn't grow. We need to redo this alongside lefty cultures in 24 well liquid plates with >2 mL of culture | |||
==[[User:Tim Hsiau|Tim Hsiau]] 19:49, 1 April 2009 (EDT)== | |||
Did 2ab reaction today. | |||
Cut with enzymes, then put in ligase mix. | |||
Transformed into Lefty and Righty cells and tomorrow we will pick 4 clones each and put into 2 96-well genetix plates filled with 8% glycerol LB. After letting them grow for a while, use the pin tool to spot onto big petri dishes. 1 LB only, 1 KCA (to check for cotransformations) | |||
Latest revision as of 16:49, 1 April 2009
~~!~~
Notes
to do
Tim Hsiau 19:33, 10 February 2009 (EST)
Grading project balls output!
Tim Hsiau 23:06, 11 February 2009 (EST)
Found site to do genomic in silico PCR http://insilico.ehu.es/PCR/ Prepro prediction http://www.cbs.dtu.dk/services/SignalP/
Tim Hsiau 18:24, 24 March 2009 (EDT)
--Use 20 tips on MP20! Diluted 3 microliter in 50 microliter water. --want to do transformations
--plating on LB agar -> transposed
Tim Hsiau 14:23, 25 March 2009 (EDT)
Having a layout conducive to multichannel pipetting is much more important than "saving wells"
Need to put > 1.5 mL of LB agar into blocks, otherwise the agar shrinks
The robot shouldn't dip into the well so much when pipetting antiobiotics. Currently, it is touching the walls of the well and might be picking up other antibiotics and cross contaminating the stock solutions
Tim Hsiau 13:57, 26 March 2009 (EDT)
Plated 20µL of culture, this amount seems to work quite well on the 24 well agar LB plates
Some righty liquid cultures didn't grow. We need to redo this alongside lefty cultures in 24 well liquid plates with >2 mL of culture
Tim Hsiau 19:49, 1 April 2009 (EDT)
Did 2ab reaction today.
Cut with enzymes, then put in ligase mix.
Transformed into Lefty and Righty cells and tomorrow we will pick 4 clones each and put into 2 96-well genetix plates filled with 8% glycerol LB. After letting them grow for a while, use the pin tool to spot onto big petri dishes. 1 LB only, 1 KCA (to check for cotransformations)
