SBB09Ntbk-SamNg: Difference between revisions
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For Monday Proceed With: | For Monday Proceed With: | ||
- EspP Autotransporter - | - EspP Autotransporter - Sequencing | ||
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Revision as of 13:55, 4 March 2009
~~Protocols~~
~~*Sam Ng 3 March 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Digest
Myc_Tag - LR Gateway Transfer
Myc_Tag - Transformation by Heat Shock
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Sequencing
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Picking of colonies
EspP Autotransporter - Miniprep purification of DNA
Additional Notes:
Went into Restriction Digestion Check, but incorrectly used all 5 ul, immediately stopped reaction and proceeded with Zymo Cleanup.
For Wednesday Proceed With: - EspP Autotransporter - Restriction Digest Check (8 ul)
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Ligation of EcoRI/BamHI digests
EspP Autotransporter - Transformation by heat-shock
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Picking of colonies
~~*Sam Ng 25 February 2009:~~
Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)
Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**
For Friday Proceed With: - EspP Autotransporter - Ligation of EcoRI/BamHI digests
~~*Sam Ng 23 February 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)
Additional Notes:
Lane 3 (~1.5kb)
[[1]]
Regular Zymo Cleanup (33ul)
For Wednesday Proceed With: - EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
~~*Sam Ng 18 February 2009:~~
Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR
Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol
Ran PCR with EHEC
For Monday Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)