SBB09Ntbk-SamNg: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 1: Line 1:
== '''Protocols''' ==
[http://openwetware.org/wiki/EspP_Autotransporter EspP_Autotransporter]<br>
[http://openwetware.org/wiki/EspP_Autotransporter EspP_Autotransporter]<br>
[http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br>
[http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br>
~~ Under Construction ~~
 
==~~*'''[[User:Sam Ng|Sam Ng]] 25 February 2009''':~~==
'''Completed Protocols:''' <br>
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products <br>
EspP Autotransporter - Regular Zymo Cleanup (2) <br>
 
'''Additional Notes:''' <br>
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**
 
<pre>
For Friday Proceed With:
- EspP Autotransporter - Ligation of EcoRI/BamHI digests
</pre>


==~~*'''[[User:Sam Ng|Sam Ng]] 23 February 2009''':~~==
==~~*'''[[User:Sam Ng|Sam Ng]] 23 February 2009''':~~==

Revision as of 13:40, 25 February 2009

Protocols

EspP_Autotransporter
Myc_Tag

~~*Sam Ng 25 February 2009:~~

Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)

Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**

For Friday Proceed With:
- EspP Autotransporter - Ligation of EcoRI/BamHI digests

~~*Sam Ng 23 February 2009:~~

Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)

Additional Notes:
Lane 3 (~1.5kb) [[1]]

Regular Zymo Cleanup (33ul)

For Wednesday Proceed With:
- EspP Autotransporter - EcoRI/BamHI Digest of PCR Products

~~*Sam Ng 18 February 2009:~~

Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR

Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol

Ran PCR with EHEC

For Monday Proceed With: 
- EspP Autotransporter - Analytical Gel
- EspP Autotransporter - Regular Zymo Cleanup (1)