SBB09Ntbk-SamNg: Difference between revisions
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==~~'''Protocols'''~~== | |||
[http://openwetware.org/wiki/EspP_Autotransporter EspP_Autotransporter]<br> | |||
[http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 9 March 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
Myc_Tag - Analytical Digest <br> | |||
Myc_Tag - Sequencing <br> | |||
'''Additional Notes:''' <br> | |||
N/A | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 6 March 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Sequencing <br> | |||
Myc_Tag - Picking of colonies <br> | |||
Myc_Tag - Miniprep purification of DNA <br> | |||
'''Additional Notes:''' <br> | |||
N/A | |||
<pre> | |||
For Monday Proceed With: | |||
- Myc_Tag - Restriction Digest Check | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 4 March 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Analytical Digest <br> | |||
Myc_Tag - LR Gateway Transfer<br> | |||
Myc_Tag - Transformation by Heat Shock<br> | |||
'''Additional Notes:''' <br> | |||
N/A | |||
<pre> | |||
For Monday Proceed With: | |||
- EspP Autotransporter - Sequencing | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 27 February 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Picking of colonies <br> | |||
EspP Autotransporter - Miniprep purification of DNA <br> | |||
'''Additional Notes:''' <br> | |||
Went into Restriction Digestion Check, but incorrectly used all 5 ul, immediately stopped reaction and proceeded with Zymo Cleanup. <br> | |||
<pre> | |||
For Wednesday Proceed With: | |||
- EspP Autotransporter - Restriction Digest Check (8 ul) | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 27 February 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Ligation of EcoRI/BamHI digests <br> | |||
EspP Autotransporter - Transformation by heat-shock <br> | |||
'''Additional Notes:''' <br> | |||
N/A | |||
<pre> | |||
For Monday Proceed With: | |||
- EspP Autotransporter - Picking of colonies | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 25 February 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products <br> | |||
EspP Autotransporter - Regular Zymo Cleanup (2) <br> | |||
'''Additional Notes:''' <br> | |||
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion** | |||
<pre> | |||
For Friday Proceed With: | |||
- EspP Autotransporter - Ligation of EcoRI/BamHI digests | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 23 February 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Analytical Gel <br> | |||
EspP Autotransporter - Regular Zymo Cleanup (1) <br> | |||
'''Additional Notes:''' <br> | |||
Lane 3 (~1.5kb) | |||
[[http://openwetware.org/wiki/Image:IMG_1608.JPG]] | |||
Regular Zymo Cleanup (33ul) | |||
<pre> | |||
For Wednesday Proceed With: | |||
- EspP Autotransporter - EcoRI/BamHI Digest of PCR Products | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 18 February 2009''':~~== | ==~~*'''[[User:Sam Ng|Sam Ng]] 18 February 2009''':~~== | ||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Oligos <br> | |||
EspP Autotransporter - Cloning by PCR <br> | |||
'''Additional Notes:''' <br> | |||
Received oligos Osn0001-F and Osn0002-R: <br> | |||
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol <br> | |||
Ran PCR with EHEC <br> | |||
<pre> | <pre> | ||
For Monday: | For Monday Proceed With: | ||
- Analytical Gel | - EspP Autotransporter - Analytical Gel | ||
- Cleanup | - EspP Autotransporter - Regular Zymo Cleanup (1) | ||
</pre> | </pre> |
Latest revision as of 11:53, 9 March 2009
~~Protocols~~
~~*Sam Ng 9 March 2009:~~
Completed Protocols:
Myc_Tag - Analytical Digest
Myc_Tag - Sequencing
Additional Notes:
N/A
~~*Sam Ng 6 March 2009:~~
Completed Protocols:
EspP Autotransporter - Sequencing
Myc_Tag - Picking of colonies
Myc_Tag - Miniprep purification of DNA
Additional Notes:
N/A
For Monday Proceed With: - Myc_Tag - Restriction Digest Check
~~*Sam Ng 4 March 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Digest
Myc_Tag - LR Gateway Transfer
Myc_Tag - Transformation by Heat Shock
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Sequencing
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Picking of colonies
EspP Autotransporter - Miniprep purification of DNA
Additional Notes:
Went into Restriction Digestion Check, but incorrectly used all 5 ul, immediately stopped reaction and proceeded with Zymo Cleanup.
For Wednesday Proceed With: - EspP Autotransporter - Restriction Digest Check (8 ul)
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Ligation of EcoRI/BamHI digests
EspP Autotransporter - Transformation by heat-shock
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Picking of colonies
~~*Sam Ng 25 February 2009:~~
Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)
Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**
For Friday Proceed With: - EspP Autotransporter - Ligation of EcoRI/BamHI digests
~~*Sam Ng 23 February 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)
Additional Notes:
Lane 3 (~1.5kb)
[[1]]
Regular Zymo Cleanup (33ul)
For Wednesday Proceed With: - EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
~~*Sam Ng 18 February 2009:~~
Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR
Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol
Ran PCR with EHEC
For Monday Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)