SBB09Ntbk-SamNg

~~Protocols~~

EspP_Autotransporter
Myc_Tag

~~*Sam Ng 4 March 2009:~~

Completed Protocols:
EspP Autotransporter - Sequencing
Myc_Tag - Picking of colonies
Myc_Tag - Miniprep purification of DNA

Additional Notes:
N/A

For Monday Proceed With:
- Myc_Tag - Restriction Digest Check

~~*Sam Ng 4 March 2009:~~

Completed Protocols:
EspP Autotransporter - Analytical Digest
Myc_Tag - LR Gateway Transfer
Myc_Tag - Transformation by Heat Shock

Additional Notes:
N/A

For Monday Proceed With:
- EspP Autotransporter - Sequencing

~~*Sam Ng 27 February 2009:~~

Completed Protocols:
EspP Autotransporter - Picking of colonies
EspP Autotransporter - Miniprep purification of DNA

Additional Notes:
Went into Restriction Digestion Check, but incorrectly used all 5 ul, immediately stopped reaction and proceeded with Zymo Cleanup.

For Wednesday Proceed With:
- EspP Autotransporter - Restriction Digest Check (8 ul)

~~*Sam Ng 27 February 2009:~~

Completed Protocols:
EspP Autotransporter - Ligation of EcoRI/BamHI digests
EspP Autotransporter - Transformation by heat-shock

Additional Notes:
N/A

For Monday Proceed With:
- EspP Autotransporter - Picking of colonies

~~*Sam Ng 25 February 2009:~~

Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)

Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**

For Friday Proceed With:
- EspP Autotransporter - Ligation of EcoRI/BamHI digests

~~*Sam Ng 23 February 2009:~~

Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)

Additional Notes:
Lane 3 (~1.5kb) [[1]]

Regular Zymo Cleanup (33ul)

For Wednesday Proceed With:
- EspP Autotransporter - EcoRI/BamHI Digest of PCR Products

~~*Sam Ng 18 February 2009:~~

Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR

Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol

Ran PCR with EHEC

For Monday Proceed With: 
- EspP Autotransporter - Analytical Gel
- EspP Autotransporter - Regular Zymo Cleanup (1)