SBB09Ntbk-SamNg: Difference between revisions
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[http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br> | [http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br> | ||
==~~*'''[[User:Sam Ng|Sam Ng]] | ==~~*'''[[User:Sam Ng|Sam Ng]] 3 March 2009''':~~== | ||
'''Completed Protocols:''' <br> | '''Completed Protocols:''' <br> | ||
EspP Autotransporter - Analytical Digest <br> | EspP Autotransporter - Analytical Digest <br> |
Revision as of 13:52, 4 March 2009
~~Protocols~~
~~*Sam Ng 3 March 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Digest
Myc_Tag - LR Gateway
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Restriction Digest Check (8 ul)
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Picking of colonies
EspP Autotransporter - Miniprep purification of DNA
Additional Notes:
Went into Restriction Digestion Check, but incorrectly used all 5 ul, immediately stopped reaction and proceeded with Zymo Cleanup.
For Wednesday Proceed With: - EspP Autotransporter - Restriction Digest Check (8 ul)
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Ligation of EcoRI/BamHI digests
EspP Autotransporter - Transformation by heat-shock
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Picking of colonies
~~*Sam Ng 25 February 2009:~~
Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)
Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**
For Friday Proceed With: - EspP Autotransporter - Ligation of EcoRI/BamHI digests
~~*Sam Ng 23 February 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)
Additional Notes:
Lane 3 (~1.5kb)
[[1]]
Regular Zymo Cleanup (33ul)
For Wednesday Proceed With: - EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
~~*Sam Ng 18 February 2009:~~
Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR
Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol
Ran PCR with EHEC
For Monday Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)