SBB09Ntbk-SamNg: Difference between revisions
No edit summary |
No edit summary |
||
Line 2: | Line 2: | ||
[http://openwetware.org/wiki/EspP_Autotransporter EspP_Autotransporter]<br> | [http://openwetware.org/wiki/EspP_Autotransporter EspP_Autotransporter]<br> | ||
[http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br> | [http://openwetware.org/wiki/Myc_Tag Myc_Tag]<br> | ||
==~~*'''[[User:Sam Ng|Sam Ng]] 27 February 2009''':~~== | |||
'''Completed Protocols:''' <br> | |||
EspP Autotransporter - Ligation of EcoRI/BamHI digests <br> | |||
EspP Autotransporter - Transformation by heat-shock <br> | |||
'''Additional Notes:''' <br> | |||
N/A | |||
<pre> | |||
For Monday Proceed With: | |||
- EspP Autotransporter - Picking of colonies | |||
</pre> | |||
==~~*'''[[User:Sam Ng|Sam Ng]] 25 February 2009''':~~== | ==~~*'''[[User:Sam Ng|Sam Ng]] 25 February 2009''':~~== |
Revision as of 13:12, 27 February 2009
~~Protocols~~
~~*Sam Ng 27 February 2009:~~
Completed Protocols:
EspP Autotransporter - Ligation of EcoRI/BamHI digests
EspP Autotransporter - Transformation by heat-shock
Additional Notes:
N/A
For Monday Proceed With: - EspP Autotransporter - Picking of colonies
~~*Sam Ng 25 February 2009:~~
Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)
Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**
For Friday Proceed With: - EspP Autotransporter - Ligation of EcoRI/BamHI digests
~~*Sam Ng 23 February 2009:~~
Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)
Additional Notes:
Lane 3 (~1.5kb)
[[1]]
Regular Zymo Cleanup (33ul)
For Wednesday Proceed With: - EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
~~*Sam Ng 18 February 2009:~~
Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR
Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol
Ran PCR with EHEC
For Monday Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)