SBB09Ntbk-SamNg

~~*Protocols:~~

EspP_Autotransporter
Myc_Tag

~~*Sam Ng 25 February 2009:~~

Completed Protocols:
EspP Autotransporter - EcoRI/BamHI Digest of PCR Products
EspP Autotransporter - Regular Zymo Cleanup (2)

Additional Notes:
Regular Zymo Cleanup (33ul) **Should have been 8ul the volume of digestion**

For Friday Proceed With:
- EspP Autotransporter - Ligation of EcoRI/BamHI digests

~~*Sam Ng 23 February 2009:~~

Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)

Additional Notes:
Lane 3 (~1.5kb) [[1]]

Regular Zymo Cleanup (33ul)

For Wednesday Proceed With:
- EspP Autotransporter - EcoRI/BamHI Digest of PCR Products

~~*Sam Ng 18 February 2009:~~

Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR

Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol

Ran PCR with EHEC

For Monday Proceed With: 
- EspP Autotransporter - Analytical Gel
- EspP Autotransporter - Regular Zymo Cleanup (1)