SBB09Ntbk-SamNg: Difference between revisions

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==~~*'''[[User:Sam Ng|Sam Ng]] 23 February 2009''':~~==
==~~*'''[[User:Sam Ng|Sam Ng]] 23 February 2009''':~~==
Ran Analytical Gel (33ul)
Zymo Cleanup (33ul)
<pre>
For Wednesday:
- Digestion
</pre>
'''Completed Protocols:''' <br>
'''Completed Protocols:''' <br>
EspP Autotransporter - Analytical Gel <br>
EspP Autotransporter - Analytical Gel <br>

Revision as of 13:36, 25 February 2009

EspP_Autotransporter
Myc_Tag
~~ Under Construction ~~

~~*Sam Ng 23 February 2009:~~

Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)

Additional Notes:
Lane 3:

Regular Zymo Cleanup (33ul)

For Wednesday Proceed With:
- EspP Autotransporter - EcoRI/BamHI Digest of PCR Products

~~*Sam Ng 18 February 2009:~~

Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR

Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol

Ran PCR with EHEC

For Monday Proceed With: 
- EspP Autotransporter - Analytical Gel
- EspP Autotransporter - Regular Zymo Cleanup (1)