SBB09Ntbk-SamNg: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 40: | Line 40: | ||
Ran PCR with EHEC <br> | Ran PCR with EHEC <br> | ||
<pre> | <pre> | ||
For Monday: | For Monday Proceed With: | ||
- EspP Autotransporter - Analytical Gel | - EspP Autotransporter - Analytical Gel | ||
- EspP Autotransporter - Regular Zymo Cleanup (1) | - EspP Autotransporter - Regular Zymo Cleanup (1) | ||
</pre> | </pre> | ||
Revision as of 13:35, 25 February 2009
EspP_Autotransporter
Myc_Tag
~~ Under Construction ~~
~~*Sam Ng 23 February 2009:~~
Ran Analytical Gel (33ul)
Zymo Cleanup (33ul)
For Wednesday: - Digestion
Completed Protocols:
EspP Autotransporter - Analytical Gel
EspP Autotransporter - Regular Zymo Cleanup (1)
Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol
Ran PCR with EHEC
For Wednesday: Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)
~~*Sam Ng 18 February 2009:~~
Completed Protocols:
EspP Autotransporter - Oligos
EspP Autotransporter - Cloning by PCR
Additional Notes:
Received oligos Osn0001-F and Osn0002-R:
Osn0001 - 27.00 nmol and Osn0002 - 23.60 nmol
Ran PCR with EHEC
For Monday Proceed With: - EspP Autotransporter - Analytical Gel - EspP Autotransporter - Regular Zymo Cleanup (1)
