SBB09Ntbk-Nikhil Sharma

Nikhil Sharma 12:18, 23 February 2009 (PST)

Today, I digested my wobble product for Poly_bind basic part. First, I ran a small fragment zymo cleanup, followed by a digestion with EcoR1/BamH1, and then another zymo clean-up. For the TraT part, I was able to do an analytical gel to determine that my product was correct. I ran a normal zymo cleanup, but I ran out of time to run the digest. I froze my eluted DNA and will digest it on Wednesday.

Nikhil Sharma 12:31, 25 February 2009 (PST)

Today I took the cleaned-up wobble digest for the Poly_bind basic part and ligated it with its vector, pBca9495AK-Bca1144#5. After following the ligation protocol, I transformed the ligated product by heat shock and plated it onto an AK plate.

For the TraT part, I was able to digest and cleanup, but I did not have enough time to ligate it. I will take care of that in the next lab period.