SBB09Ntbk-Nikhil Sharma: Difference between revisions
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For the TraT part, I was able to digest and cleanup, but I did not have enough time to ligate it. I will take care of that in the next lab period. | For the TraT part, I was able to digest and cleanup, but I did not have enough time to ligate it. I will take care of that in the next lab period. | ||
==[[User: Nikhil Sharma|Nikhil Sharma]] 1:31, 27 February 2009 (PST)== | |||
Today, I did a miniprep purification of the transformed Poly_bind basic part. I had enough colonies on my plate to suggest transformation had occurred. I wasn't able to do the mapping at the end of the purification, so I will do that on Monday. | |||
I was able to transform the TraT part, and I will proceed to miniprep purify it on Monday. |
Revision as of 11:20, 2 March 2009
Nikhil Sharma 12:18, 23 February 2009 (PST)
Today, I digested my wobble product for Poly_bind basic part. First, I ran a small fragment zymo cleanup, followed by a digestion with EcoR1/BamH1, and then another zymo clean-up. For the TraT part, I was able to do an analytical gel to determine that my product was correct. I ran a normal zymo cleanup, but I ran out of time to run the digest. I froze my eluted DNA and will digest it on Wednesday.
Nikhil Sharma 12:31, 25 February 2009 (PST)
Today I took the cleaned-up wobble digest for the Poly_bind basic part and ligated it with its vector, pBca9495AK-Bca1144#5. After following the ligation protocol, I transformed the ligated product by heat shock and plated it onto an AK plate.
For the TraT part, I was able to digest and cleanup, but I did not have enough time to ligate it. I will take care of that in the next lab period.
Nikhil Sharma 1:31, 27 February 2009 (PST)
Today, I did a miniprep purification of the transformed Poly_bind basic part. I had enough colonies on my plate to suggest transformation had occurred. I wasn't able to do the mapping at the end of the purification, so I will do that on Monday.
I was able to transform the TraT part, and I will proceed to miniprep purify it on Monday.