SBB09Ntbk-Nikhil Sharma: Difference between revisions
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(New page: ==Nikhil Sharma 12:18, 23 February 2009 (PST)== Today, I digested my wobble product for Poly_bind basic part. First, I ran a small fragment zymo cleanup, followed b...) |
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Revision as of 13:21, 23 February 2009
Nikhil Sharma 12:18, 23 February 2009 (PST)
Today, I digested my wobble product for Poly_bind basic part. First, I ran a small fragment zymo cleanup, followed by a digestion with EcoR1/BamH1, and then another zymo clean-up. For the TraT part, I was able to do an analytical gel to determine that my product was correct. I ran a normal zymo cleanup, but I ran out of time to run the digest. I froze my eluted DNA and will digest it on Wednesday.
