SBB09Ntbk-Kamran Ali: Difference between revisions
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== '''2-27-2009''' == | |||
Reran the products in an analytical gel but the PCR products themselves were somehow lost/misplaced.<br> Repeated the 2 PCR reactions from 2/25 and at 45 C annealing temperature | |||
== '''2-27-2009''' == | |||
Ran the products in an analytical gel but the gel was poor and all the columns including the ladder had smears. | |||
== '''2-25-2009''' == | == '''2-25-2009''' == | ||
Prepared 2 new PCR reactions of my oligos as in 2/18 but these reactions were run at a 50 C annealing temperature. | Prepared 2 new PCR reactions of my oligos as in 2/18 (1 w/DMSO & 1 w/o) but these reactions were run at a 50 C annealing temperature. | ||
Also DMSO was added to one of the reactions. | Also DMSO was added to one of the reactions. | ||
== '''2-23-2009''' == | == '''2-23-2009''' == | ||
Revision as of 14:22, 2 March 2009
2-27-2009
Reran the products in an analytical gel but the PCR products themselves were somehow lost/misplaced.
Repeated the 2 PCR reactions from 2/25 and at 45 C annealing temperature
2-27-2009
Ran the products in an analytical gel but the gel was poor and all the columns including the ladder had smears.
2-25-2009
Prepared 2 new PCR reactions of my oligos as in 2/18 (1 w/DMSO & 1 w/o) but these reactions were run at a 50 C annealing temperature. Also DMSO was added to one of the reactions.
2-23-2009
Ran the PCR product in an analytical gel but no band appeared so the reaction failed
2-18-2009
Got the oligos and ran the reaction:
PCR Ost005F and Ost006R on E. coli avian APEC genomic DNA
