SBB09Ntbk-Jong H. Shin: Difference between revisions
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<br>mine is the 5th one<br> | <br>mine is the 5th one<br> | ||
The size is between 1.5k and 1k; it's about the right size I've expected. Product? Yes.<br> | The size is between 1.5k and 1k; it's about the right size I've expected. Product? Yes.<br> | ||
[[http://openwetware.org/images/f/f2/IMG_1608.JPG The picture link for the second product] mine is the 2nd one<br> | [[http://openwetware.org/images/f/f2/IMG_1608.JPG The picture link for the second product]<br> | ||
mine is the 2nd one<br> | |||
The result came out the same as the first one. Product? Yes!<br> | The result came out the same as the first one. Product? Yes!<br> | ||
7. Zymo cleanup<br> | 7. Zymo cleanup<br> | ||
Revision as of 13:38, 23 February 2009
Feb 18 2009
First day of the actual lab.
Lab work on my project.
Template:SBB09 19329
upaG Long
I added 275μL of water in the given tube in which JS001F oligo was and 343μL of water in the JS002R tube.
Then, I followed the instruction below.
Cloning by PCR
Since the size is over 4000K, I put the PCR tube in 8k55.
Feb 23 2009
---
Second day of the lab
I guess Prof. Chris forgot to give me oligos for Alkaline Phosphatase
I should do it later once i get the oligos
To decide if there's product in the PCR tube I worked on Feb 18
I need to run it on gel
1. Add 5μL of the product and 1μL of loading buffer
2. Insert 6μL in total onto gel
3. Wait for about 10 minutes
4. GSIs take a picture of my analytical gel
6. The picture link for the first product
mine is the 5th one
The size is between 1.5k and 1k; it's about the right size I've expected. Product? Yes.
[The picture link for the second product
mine is the 2nd one
The result came out the same as the first one. Product? Yes!
7. Zymo cleanup
