SBB09Ntbk-Hank Shih: Difference between revisions
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Bhs003R Reverse BamHI for a~eaeA_Display> GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC | Bhs003R Reverse BamHI for a~eaeA_Display> GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC | ||
</pre> | </pre> | ||
'''02/18/2009'''<br> | |||
Today, I've setup my first two PCR reactions with Bhs001F/Bhs002R and Bhs002F/Bhs003R. | |||
Revision as of 12:16, 25 February 2009
02/09/2009
Finished making basic part for M10024. This is the final construction file:
Construction of {a~eaeA_Display>} basic part
PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A)
PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B)
----------------------------
PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI)
Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10024 {a~eaeA_Display>}
----------------------------
Bhs001F Forward EcoRI for a~eaeA_Display> cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG
Bhs002F Removing the EcoRI site from eaeA_Display GTTAATCAGAACTCATTTGCAAATGG
Bhs002R Removing the EcoRI site from eaeA_Display CCATTTGCAAATGAGTTCTGATTAAC
Bhs003R Reverse BamHI for a~eaeA_Display> GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC
02/18/2009
Today, I've setup my first two PCR reactions with Bhs001F/Bhs002R and Bhs002F/Bhs003R.
