SBB09Ntbk-DaviddeRenzy: Difference between revisions
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== [[User:David De Renzy|David De Renzy]] 14:53, 18 February 2009 (EST) == | == [[User:David De Renzy|David De Renzy]] 14:53, 18 February 2009 (EST) == | ||
Reconstituted oligos to 100μM | |||
Ran basic PCR cloning reaction | Ran basic PCR cloning reaction | ||
PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI) | PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI) | ||
Into PCR tube labeled M10040 | |||
Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg | Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg | ||
Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg | Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg | ||
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PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) | PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) | ||
Into PCR tube labeled M10041 | Into PCR tube labeled M10041 | ||
Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | ||
BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc | BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc | ||
<pre> | <pre> | ||
Next step - Run Analytical Gel | Next step -> Run Analytical Gel | ||
</pre> | </pre> | ||
Revision as of 12:59, 18 February 2009
David De Renzy 14:34, 4 February 2009 (EST)
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David De Renzy 14:53, 18 February 2009 (EST)
Reconstituted oligos to 100μM Ran basic PCR cloning reaction PCR Odd001F/Odd002R on E. Coli plasmid pAPEC-1 (AF218073) (1603 bp, EcoRI/BamHI) Into PCR tube labeled M10040 Odd001F Forward EcoRI oligo for TshA ccataGAATTCatgAGATCTacacttttacctgtatacg Odd002R Reverse BamHI oligo for TshA ctgatGGATCCtcagaatgaataacgaatattagcg
AND
PCR Odd003F/BBa_G00101 on pBca1256-Bca1346 (3722 bp, EcoRI/BamHI) Into PCR tube labeled M10041 Odd003F Forward oligo for INP ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg BBa_G00101 Reverse sequencing of pSB1A* plasmids attaccgcctttgagtgagc
Next step -> Run Analytical Gel
