Wayne G. Shreffler at 01:16, 28 May 2009

Wayne G. Shreffler — Thu, 28 May 2009 01:16:13 GMT

←Older revision		Revision as of 01:16, 28 May 2009
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	* 38.17 g Sodium Borate decahydrate		* 38.17 g Sodium Borate decahydrate
	* 33 g Boric Acid		* 33 g Boric Acid
-	* Bring to 1L with dH<sub>2</sub>O)	+	* Bring to 1L with dH<sub>2</sub>O

	* Dilute to 1X and use to make gel and running buffer.		* Dilute to 1X and use to make gel and running buffer.

Jonathan Lake at 00:05, 7 April 2009

Jonathan Lake — Tue, 07 Apr 2009 00:05:52 GMT

←Older revision		Revision as of 00:05, 7 April 2009
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	[[Category:In vitro]]		[[Category:In vitro]]
	[[Category:DNA]]		[[Category:DNA]]
-	[[Category:Agarose ~~Gel Electrophoresis~~]]	+	[[Category:Agarose gel electrophoresis]]
	[[Category:Material]]		[[Category:Material]]

Jonathan Lake at 00:05, 7 April 2009

Jonathan Lake — Tue, 07 Apr 2009 00:05:09 GMT

←Older revision		Revision as of 00:05, 7 April 2009
Line 44:		Line 44:
	[[Category:In vitro]]		[[Category:In vitro]]
	[[Category:DNA]]		[[Category:DNA]]
-	[[Category:Agarose Gel ~~Electropheresis~~]]	+	[[Category:Agarose Gel Electrophoresis]]
	[[Category:Material]]		[[Category:Material]]

Jonathan Lake: New page: ==Curators== ''Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is cu...

Jonathan Lake — Tue, 07 Apr 2009 00:03:17 GMT

New page: ==Curators== ''Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is cu...

New page

==Curators==
''Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently [[OpenWetWare:Information management/Protocol curators|discussing the idea of protocol curators]]. Please contribute.''

==Abstract==

SB (Sodium Borate or Sodium Boric Acid) buffer is a agarose gel electropheresis buffer for DNA gels. It has low conductivity and allows for less heat buildup and thus higher voltage and faster runs.

===Reagents===
* Sodium Borate decahydrate (Borax)
* Boric Acid
* dH<sub>2</sub>O

==Procedure==
A simple version of this buffer can be easily made as a 20X (100 mM) concentrate.

* 38.17 g Sodium Borate decahydrate
* 33 g Boric Acid
* Bring to 1L with dH<sub>2</sub>O)

* Dilute to 1X and use to make gel and running buffer.

==Troubleshooting==
There are some caveats here. Loading DNA that is in a high-salt solution (some DNA ladders, loading dyes, or restriction enzyme buffers) can increase the local conductivity around the sample and change its running characteristics - meaning that samples in different buffers won't always run at the same speed. The quickest solution here is actually to dilute the sample to the largest volume that you can load in the well.

In addition, one should minimize the amount of indicator dye in the loading dye, as this is a salt and contributes significantly to this problem. Using a fainter dye helps to increase the resolution of these gels.
==Notes==

It should be noted that there are several other "next-gen" electropheresis buffers, notably [[LA]] - Lithium acetate, which is touted as being superior in many respects to SB.

==References==

<biblio>
#Brody-Kern pmid=14989083
#website http://rrresearch.blogspot.com/2007/03/running-gels.html
//"Easy Version"
</biblio>

==Discussion==
You can [[Talk:{{PAGENAME}}|discuss this protocol]].

[[Category:Protocol]]
[[Category:In vitro]]
[[Category:DNA]]
[[Category:Agarose Gel Electropheresis]]
[[Category:Material]]

SB - Revision history

Wayne G. Shreffler at 01:16, 28 May 2009

Jonathan Lake at 00:05, 7 April 2009

Jonathan Lake at 00:05, 7 April 2009

Jonathan Lake: New page: ==Curators== ''Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is cu...