Robert W Arnold Week 12: Difference between revisions

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===Week 12 Assignment===
===Week 12 Assignment===
#What samples did they collect and use for the microarray experiment?
#What samples did they collect and use for the microarray experiment?
#*
#*3 replicates of control culture and ranalexin were used in the microarray experiment.  6 total arrays were used in analysis.
#How many microarray chips did they hybridize in the experiment?
#How many microarray chips did they hybridize in the experiment?
#*
#*2 microarray chips were used.
#Which samples were paired to hybridize on the chip?
#Which samples were paired to hybridize on the chip?
#*
#*Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
#Which was labeled red (Cy5)?  Which was labeled green (Cy3)?
#Which was labeled red (Cy5)?  Which was labeled green (Cy3)?
#*
#*Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5).  Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
#How many replicates did they perform of each type?
#How many replicates did they perform of each type?
#*
#*2 technical replicates of each type.
 
*Arrary data file names
**Ranalexin (A1) - MBAD_12054.txt
**Ranalexin (A2) - MBAD_12061.txt
**MRSA-252 (A1) - MBAD_12055.txt
**MRSA-252 (A2) - MBAD_12060.txt


==Links==
==Links==

Revision as of 16:17, 29 November 2011

Electronic Lab Notebook 12

Robert W Arnold

Week 12 Assignment

Journal Club

  • Finished up journal club.
  • Brought up microarray data from MRSA paper.
  • Downloaded microarray data.

Uploaded Data

Experiment E-BUG Data

Array Data

Week 12 Assignment

  1. What samples did they collect and use for the microarray experiment?
    • 3 replicates of control culture and ranalexin were used in the microarray experiment. 6 total arrays were used in analysis.
  2. How many microarray chips did they hybridize in the experiment?
    • 2 microarray chips were used.
  3. Which samples were paired to hybridize on the chip?
    • Ranalexin (A1) and MRSA-252(A2). MRSA-252(A1) and Ranalexin(A2).
  4. Which was labeled red (Cy5)? Which was labeled green (Cy3)?
    • Ranalexin (A1) and MRSA-252(A2) was labeled red (Cy5). Ranalexin (A2) and MRSA-252(A1) was labeled green (Cy3).
  5. How many replicates did they perform of each type?
    • 2 technical replicates of each type.
  • Arrary data file names
    • Ranalexin (A1) - MBAD_12054.txt
    • Ranalexin (A2) - MBAD_12061.txt
    • MRSA-252 (A1) - MBAD_12055.txt
    • MRSA-252 (A2) - MBAD_12060.txt

Links

Biol 368 Homepage

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