Richard Lab:protocols: Difference between revisions
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Back to [[Richard_Lab | Richard Lab]] | Back to [[Richard_Lab | Richard Lab Main Page]] | ||
<center> | <center> | ||
=Equipment= | =Equipment= | ||
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::'''[[Richard Lab:ASE overview|Overview]]''' | ::'''[[Richard Lab:ASE overview|Overview]]''' | ||
==Anaerobic Chamber== | ==Anaerobic Chamber== | ||
::'''[[Richard Lab:anaerobic chamber|Overview]]''' | |||
==YSI Analyzer== | |||
::'''[[YSI 2700 Select Biochemistry Analyzer|Overview]] | |||
::'''[[Quantification of glucose]] | |||
<center> | <center> | ||
=Methods= | =Methods= | ||
</center> | </center> | ||
==Biomass Protocols== | |||
::'''[[Silage protocols|Silage Protocols]]''' | |||
::'''[[Biomass compositional analysis|Biomass Compositional Analysis]]''' | |||
::'''[[Richard_Lab:_NDF|NDF (Neutral Detergent Fiber) Determination]]''' | |||
::'''[[Richard_Lab:ADF|ADF (Acid Detergent Fiber) Determination]]''' | |||
::'''[[Richard_Lab:ADL|ADL (Acid Detergent Lignin) Determination]]''' | |||
::'''[[Richard Lab:Dilute-acid pretreatment|Dilute-Acid Pretreatment of Biomass]]''' | |||
::'''[[Richard Lab:Enzymatic Hydrolysis of Biomass|Enzymatic Hydrolysis of Biomass]]''' | |||
::'''[[Quantification of glucose|Quantification of Glucose]]''' | |||
::'''[[Richard Lab:SSCF|Simultaneous Saccharification and Co-Fermentation (SSCF)]]''' | |||
::'''[[SSF Ethanol production]]''' | |||
::'''[[Acidification of C and N Samples using Tartic Acid]]''' | |||
==Enzymatic Screens and Assays== | |||
::'''[[Feruloyl Esterase Protocols|Ferulic Acid Esterase Protocols]]''' | |||
::'''[[Laccase Protocols]]''' | |||
::'''[[Xylanase Protocols]]''' | |||
::'''[[Beta-galactosidase assay|Beta-galactosidase (LacZ) Assay]]''' | |||
::'''[[Beta-galactosidase Screen]] | |||
::'''[[Beta-glucuronidase protocols|Beta-glucuronidase (GusA) Protocols]]''' | |||
::'''[[Cellulase assays]]''' | |||
==Molecular/Synthetic Biology== | ==Molecular/Synthetic Biology== | ||
::'''[[Richard Lab:Amplified Insert Assembly|Amplified Insert Assembly]]''' | ::'''[[Richard Lab:Amplified Insert Assembly|Amplified Insert Assembly]]''' | ||
::'''[[Richard Lab:Bio-Brick Formats|Bio-Brick Formats]]''' | ::'''[[Richard Lab:Bio-Brick Formats|Bio-Brick Formats]]''' | ||
::'''[[Richard Lab:PCR|PCR Protocols]]''' | |||
::'''Richard Lab PCR Protocols''' | |||
::'''[[Richard Lab:Site Directed Mutagenesis| Site Directed Mutagenesis]]''' | ::'''[[Richard Lab:Site Directed Mutagenesis| Site Directed Mutagenesis]]''' | ||
::'''[[Miniprep/GET_buffer | Plasmid Extraction]]''' | ::'''[[Miniprep/GET_buffer | Plasmid Extraction]]''' | ||
::'''[[DNA Quantification]]''' | ::'''[[DNA Quantification]]''' | ||
::'''[[Ethanol_precipitation_of_nucleic_acids | DNA Concentration]]''' | ::'''[[Ethanol_precipitation_of_nucleic_acids | DNA Concentration]]''' | ||
::'''[[DNA Synthesis from Oligos|DNA Synthesis]]''' | ::'''[[DNA Synthesis from Oligos|DNA Synthesis]]''' | ||
::'''[[Richard_Lab:Restriction_Digest | Restriction Digestion]]''' | ::'''[[Richard_Lab:Restriction_Digest | Restriction Digestion]]''' | ||
::'''[[Partial Restriction Digest]]''' | ::'''[[Partial Restriction Digest]]''' | ||
::'''[[Richard_Lab:_Agarose_Gel_Electrophoresis| Agarose Gel Electrophoresis]]''' | ::'''[[Richard_Lab:_Agarose_Gel_Electrophoresis| Agarose Gel Electrophoresis]]''' | ||
::'''[[Polyacrylamide Gel Electrophoresis]]''' | ::'''[[Polyacrylamide Gel Electrophoresis]]''' | ||
::'''[[DNA_Gel_extraction | Gel Extraction of DNA]]''' | ::'''[[DNA_Gel_extraction | Gel Extraction of DNA]]''' | ||
::'''[[Richard_Lab:Ligation|Ligation]]''' | ::'''[[Richard_Lab:Ligation|Ligation]]''' | ||
::'''[[In vitro modification of DNA for L. plantarum|Preparation of Shuttle-vectors for transformation]]''' | ::'''[[In vitro modification of DNA for L. plantarum|Preparation of Shuttle-vectors for transformation]]''' | ||
::'''[[Transforming Lactococcus lactis]]''' | |||
::'''[[Richard_Lab:Preparing_electrocompetent_cells|Making Electro-competent ''E. coli'']]''' | ::'''[[Richard_Lab:Preparing_electrocompetent_cells|Making Electro-competent ''E. coli'']]''' | ||
::'''[[Richard Lab:Electroporation of E. coli|Electro-transformation of ''E. coli'']]''' | ::'''[[Richard Lab:Electroporation of E. coli|Electro-transformation of ''E. coli'']]''' | ||
::'''[[Lactobacillus transformation (Speer 2012)|Electro-transformation of ''L. Plantarum'']]''' | |||
::'''[[ | ::'''[[Chromosomal Integration]]''' | ||
::'''Chromosomal Integration | |||
==Manure Storage and Emissions Analysis== | ==Manure Storage and Emissions Analysis== | ||
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::'''BOD, moisture and VS content''' | ::'''BOD, moisture and VS content''' | ||
:::*Organic matter samples are analyzed according to the following instructions for [http://www.abe.psu.edu/bioconversion/biofilter_characterization/BOD.htm BOD (Biological Oxygen Demand)] and [http://www.abe.psu.edu/bioconversion/biofilter_characterization/moisture_and_VS.htm moisture and Volatile Solids (organic matter) content] analysis. | :::*Organic matter samples are analyzed according to the following instructions for [http://www.abe.psu.edu/bioconversion/biofilter_characterization/BOD.htm BOD (Biological Oxygen Demand)] and [http://www.abe.psu.edu/bioconversion/biofilter_characterization/moisture_and_VS.htm moisture and Volatile Solids (organic matter) content] analysis. | ||
==Compost Analysis== | ==Compost Analysis== | ||
::'''[[Chemical Oxygen Demand]]''' | ::'''[[Chemical Oxygen Demand]]''' | ||
::'''[[Compost Pile Monitoring]]''' | |||
::'''[[CO2 Respiration]]''' | |||
::'''[[Manure and Compost Sampling Preparation Protocols]]''' | |||
::'''[[Acidification of compost with 0.5 N HCl]]''' | |||
::'''[[Free Air Space Testing Procedure]]''' | |||
Back to [[Richard_Lab | Richard Lab]] | Back to [[Richard_Lab | Richard Lab]] | ||
Latest revision as of 13:35, 10 January 2012
Back to Richard Lab Main Page
Equipment
Dionex IC
Dionex ASE
Anaerobic Chamber
YSI Analyzer
Methods
Biomass Protocols
- Silage Protocols
- Biomass Compositional Analysis
- NDF (Neutral Detergent Fiber) Determination
- ADF (Acid Detergent Fiber) Determination
- ADL (Acid Detergent Lignin) Determination
- Dilute-Acid Pretreatment of Biomass
- Enzymatic Hydrolysis of Biomass
- Quantification of Glucose
- Simultaneous Saccharification and Co-Fermentation (SSCF)
- SSF Ethanol production
- Acidification of C and N Samples using Tartic Acid
Enzymatic Screens and Assays
Molecular/Synthetic Biology
- Amplified Insert Assembly
- Bio-Brick Formats
- PCR Protocols
- Site Directed Mutagenesis
- Plasmid Extraction
- DNA Quantification
- DNA Concentration
- DNA Synthesis
- Restriction Digestion
- Partial Restriction Digest
- Agarose Gel Electrophoresis
- Polyacrylamide Gel Electrophoresis
- Gel Extraction of DNA
- Ligation
- Preparation of Shuttle-vectors for transformation
- Transforming Lactococcus lactis
- Making Electro-competent E. coli
- Electro-transformation of E. coli
- Electro-transformation of L. Plantarum
- Chromosomal Integration
Manure Storage and Emissions Analysis
- Measuring and Analyzing Gas-Flux from a Surface
- Setting Up Biofilters in the Field.
- Formatting Data from Data Logger
- The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
- BOD, moisture and VS content
- Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.
Compost Analysis
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