Richard Lab:protocols: Difference between revisions
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::'''[[Preparation of Lactobacillus Competent Cells|Making Electro-competent ''L. plantarum'']]''' | ::'''[[Preparation of Lactobacillus Competent Cells|Making Electro-competent ''L. plantarum'']]''' | ||
::'''[[Electrotransformation of Lactobacillus plantarum|Electro-transformation of ''L. Plantarum'']]''' | ::'''[[Electrotransformation of Lactobacillus plantarum|Electro-transformation of ''L. Plantarum'']]''' | ||
::'''Chromosomal Integration''' | ::'''[[Chromosomal Integration]]''' | ||
==Manure Storage and Emissions Analysis== | ==Manure Storage and Emissions Analysis== |
Revision as of 07:53, 29 March 2011
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Equipment
Dionex IC
Dionex ASE
Anaerobic Chamber
Methods
Biomass Analysis
- Mini-Silo Sampling
- NDF (Neutral Detergent Fiber)
- ADF (Acid Detergent Fiber)
- ADL (Acid Detergent Lignin)
- Acetyl Bromide Lignin Analysis
- TMAH Lignin Analysis
- Enzymatic Hydrolysis of Biomass
- Quantification of Glucose
Enzymatic Screens and Assays
Molecular/Synthetic Biology
- Amplified Insert Assembly
- Bio-Brick Formats
- Richard Lab PCR Protocols
- Site Directed Mutagenesis
- Plasmid Extraction
- DNA Quantification
- DNA Concentration
- DNA Synthesis
- Restriction Digestion
- Partial Restriction Digest
- Agarose Gel Electrophoresis
- Polyacrylamide Gel Electrophoresis
- Gel Extraction of DNA
- Ligation
- Preparation of Shuttle-vectors for transformation
- Making Electro-competent E. coli
- Electro-transformation of E. coli
- Making Electro-competent L. plantarum
- Electro-transformation of L. Plantarum
- Chromosomal Integration
Manure Storage and Emissions Analysis
- Measuring and Analyzing Gas-Flux from a Surface
- Setting Up Biofilters in the Field.
- Formatting Data from Data Logger
- The following is a set of instructions on how to use macros to transform a data set from configuration 1 to configuration 2.
- BOD, moisture and VS content
- Organic matter samples are analyzed according to the following instructions for BOD (Biological Oxygen Demand) and moisture and Volatile Solids (organic matter) content analysis.
Compost Analysis
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