Richard Lab:PCR: Difference between revisions
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===Quantification=== | ===Quantification=== | ||
*mRNA Quantification by qPCR | *mRNA Quantification by qPCR | ||
*Population Analysis by qPCR | *Population Analysis by [[Richard Lab:qPCR|qPCR]] |
Revision as of 17:14, 14 September 2011
Back to Richard Lab:protocols
Overview
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.
Amplification
- For Amplified Insert Assembly
- With Taq Polymerase
- With Vent Polymerase
- With Phusion Polymerase
DNA Synthesis
- DNA Synthesis from Oligos for making smaller parts
- PCR Overlap Extension for larger constructs from small parts.
Mutagenesis
- Richard Lab:Site Directed Mutagenesis
- Random Mutagenesis
- Saturation Mutagenesis
Quantification
- mRNA Quantification by qPCR
- Population Analysis by qPCR