Richard Lab:PCR: Difference between revisions
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While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab. | ||
===Amplification=== | ===Amplification=== | ||
*For Amplified Insert Assembly | |||
*With Taq Polymerase | |||
*With Vent Polymerase | |||
*With Phusion Polymerase | |||
===DNA Synthesis=== | ===DNA Synthesis=== | ||
*[[DNA Synthesis from Oligos]] for making smaller parts | *[[DNA Synthesis from Oligos]] for making smaller parts | ||
Revision as of 15:27, 14 September 2011
Back to Richard Lab:protocols
Overview
While PCR is typically used for amplification of DNA it can also be used for many other things. Here is a list of the PCR protocols typically used in the Richard Lab.
Amplification
- For Amplified Insert Assembly
- With Taq Polymerase
- With Vent Polymerase
- With Phusion Polymerase
DNA Synthesis
- DNA Synthesis from Oligos for making smaller parts
- PCR Overlap Extension for larger constructs from small parts.
Mutagenesis
- Richard Lab:Site Directed Mutagenesis
- Random Mutagenesis
- Saturation Mutagenesis
