Resuspension of primers

Introduction

When receiving oligonucleotide primers from a manufacturer such as Invitrogen, the oligos arrive dry and must be resuspended in buffer. The proper choice of buffer will depend on the intended application of the primers, some common ones are:

1. Sequencing/PCR
2. Annealing

Method

Invitrogen recommends the following reconstitution procedure -

• Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
• Resuspend in TE buffer, pH 8.0 at a concentration greater than 10μM.
• Allow to sit for 2mins, then vortex for 15s.