Restriction enzymes: Difference between revisions

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Restriction enzymes are used for performing a [[restriction digest]].
Restriction enzymes are used for performing a [[restriction digest]].


Comprehensive information can be found in [http://rebase.neb.com/rebase/rebase.html REBASE].
Comprehensive information can be found in [http://rebase.neb.com/rebase/rebase.html REBASE]. [[NeerajCutterList|Neeraj Dhar's good cutter list]] is a list of enzymes which are particularly reliable and straightforwards.
 
This is another source of info on restricition enzymes. It's by the same people who provide REBASE, but in a different format:
 
 
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp Activity Chart for Restriction Enzymes]
 


These wiki pages can contain people's experiences with using specific enzymes.
These wiki pages can contain people's experiences with using specific enzymes.


==Specific enzyme information==
==Specific enzyme information==
*[[DpnI]]
{{#dpl:category=Material|category=Enzyme|category=Endonuclease}}
*[[EcoRI]]
*[[PstI]]
*[[SpeI]]
*[[XbaI]]
*[[XmaI]]
 
 
===Offset Cutters===
*[[BsmBI]]


===Transmitted Wisdom===
[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]
*[[NeerajCutterList|Neeraj Dhar's good cutter list]]

Latest revision as of 06:26, 3 April 2008

General information

Restriction enzymes are used for performing a restriction digest.

Comprehensive information can be found in REBASE. Neeraj Dhar's good cutter list is a list of enzymes which are particularly reliable and straightforwards.

This is another source of info on restricition enzymes. It's by the same people who provide REBASE, but in a different format:


Activity Chart for Restriction Enzymes


These wiki pages can contain people's experiences with using specific enzymes.

Specific enzyme information