Restriction Digest with HaeIII
From OpenWetWare
Notes
- Fabric Name BsuRI
- Restictive Enzyme: BsuRI (HaeIII) store at -20°C
- Plasmid: pBSKS concentration 4μg/μL
- 20μL final volume
- add restictive enzyme to the mixture last
- after everything is mixed, incubate at 37°C for at least one hour
Experimental
- 2μL buffer R
- 3μL plasmid
- 2μL enzyme
- 13μL D H2O
Control
- 2μL buffer R
- 3μL plasmid
- 0μL enzyme (no enzyme)
- 15μL DH2O
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