Research Proposal Page: Difference between revisions
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'''Proposal Topic''': Bone Tissue Engineering | '''Proposal Topic''': Bone Tissue Engineering | ||
'''Background of problem''': A current bone tissue engineering strategy consists of growing mesenchymal stem cells on scaffolds, inducing them to differentiate into osteoblasts, and culturing these cells on the scaffold. However, this protocol is not very successful in in vivo experiments. | '''Background of problem''': A current bone tissue engineering strategy consists of growing mesenchymal stem cells on scaffolds, inducing them to differentiate into osteoblasts, and culturing these cells on the scaffold. Then, the osteoblasts will begin to deposit bone matrix, in a process known as intramembranous ossification. However, this protocol is not very successful in in vivo experiments. | ||
Therefore, researchers have begun looking into endochondral ossification, which involves the formation of cartilage. After cartilage forms, osteoblasts penetrate the cartilage matrix and begin to deposit bone matrix. | |||
However, endochondral ossification usually take up to 3-4 weeks, which is a long time. Therefore, we aim to accelerate the process of in vitro bone formation using ultrasound stimulation. | |||
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Revision as of 12:30, 3 May 2009
Mahesh Vidula and Michael Meyer 20.109 TR Red
Proposal Topic: Bone Tissue Engineering
Background of problem: A current bone tissue engineering strategy consists of growing mesenchymal stem cells on scaffolds, inducing them to differentiate into osteoblasts, and culturing these cells on the scaffold. Then, the osteoblasts will begin to deposit bone matrix, in a process known as intramembranous ossification. However, this protocol is not very successful in in vivo experiments.
Therefore, researchers have begun looking into endochondral ossification, which involves the formation of cartilage. After cartilage forms, osteoblasts penetrate the cartilage matrix and begin to deposit bone matrix.
However, endochondral ossification usually take up to 3-4 weeks, which is a long time. Therefore, we aim to accelerate the process of in vitro bone formation using ultrasound stimulation.
