Registry/Measurement kit/Notebook/Design: Difference between revisions

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==Preliminary Proposed kit designs==
==Preliminary Proposed Kit Designs==
===Promoter tester with GFP===
===Promoter tester with GFP===
*E0240 (pSB1A2)
*E0240 (pSB1A2)
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===RBS tester with RFP===
===RBS tester with RFP===
*R0040 (pSB1A2) + J04650 (pSB1AK3)
*R0040 (pSB1A2) + J04650 (pSB1AK3)
==Internal labeling system==
*1 - E0240
*2 - R0040
*3 - I13401
*4 - J04650
*5 - B0032

Revision as of 21:21, 11 June 2007

Design considerations for the expression measurement kit

Promoter

  • Constituitive?
  • Relative strength
  • Location of transcription initiation (outside of or within the promoter?)
  • Previous work

Ribosome Binding Site

  • Relative Strength
  • Previous work (<bbpart>BBa_B0032</bbpart>)

Fluorescent Protein

  • Color?
  • previous work (<bbpart>E0040</bbpart>)
  • Tagged?

Terminator

  • Do we need one?
  • Which one?
  • Previous work (<bbpart>BBa_B0015</bbpart>)


Preliminary Proposed Kit Designs

Promoter tester with GFP

  • E0240 (pSB1A2)

Promoter tester with RFP

  • B0032 (pSB1A2) + J04650 (pSB1AK3)

RBS tester with GFP

  • R0040 (pSB1A2) + I13401 (pSB1A2)

RBS tester with RFP

  • R0040 (pSB1A2) + J04650 (pSB1AK3)

Internal labeling system

  • 1 - E0240
  • 2 - R0040
  • 3 - I13401
  • 4 - J04650
  • 5 - B0032
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