Registry/Measurement kit/Notebook/2007-8-14: Difference between revisions
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(New page: *PCR cleaned NotI digests of 56 and 55 (EX, SX) *re-circularizing ligation. *Sent some sequencing requests: **E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,...) |
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*PCR cleaned NotI digests of 56 and 55 (EX, SX) | *PCR cleaned NotI digests of 56 and 55 (EX, SX) | ||
*re-circularizing ligation. | *re-circularizing ligation. | ||
*Transforming into Top10. | |||
*Made overnights of colonies expresing GFP: 55-SX-B0031, 55-SX-B43, 55-EX-B34 (2 of each) | |||
*Sent some sequencing requests: | *Sent some sequencing requests: | ||
**E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,6 | **E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,6 | ||
Latest revision as of 19:23, 14 August 2007
- PCR cleaned NotI digests of 56 and 55 (EX, SX)
- re-circularizing ligation.
- Transforming into Top10.
- Made overnights of colonies expresing GFP: 55-SX-B0031, 55-SX-B43, 55-EX-B34 (2 of each)
- Sent some sequencing requests:
- E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,6
