Registry/Measurement kit/Notebook/2007-8-1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 7: | Line 7: | ||
*Purified digests (NotI, E/X, S/X) | *Purified digests (NotI, E/X, S/X) | ||
*Re-sealed and transformed. | *Re-sealed and transformed. | ||
* | *Will test B0030, B0031, B0032, B0034 | ||
==I2055== | ==I2055== | ||
Latest revision as of 13:49, 1 August 2007
I2057
- Started digest of promoters (E/S).
- Started another culture
- To do: ligate R0040 into the digests from the PCR and transform.
I2056
- Purified digests (NotI, E/X, S/X)
- Re-sealed and transformed.
- Will test B0030, B0031, B0032, B0034
I2055
- Running gel of BioBrick PCR.
- (ladder, I2055-EX, I2055-SX)
- Both look right size.
- Restriction digests: E/X, S/X, and NotI.
T9002
- Re-did scarring PCR of F2620.
