Registry/Measurement kit/Notebook/2007-7-6: Difference between revisions
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#1uL DNA | #1uL DNA | ||
#67uL H2O | #67uL H2O | ||
*Just realized that we skipped the ligation step completely (3K3 and 1AK3). | |||
**Doing that now, discarding PCR product. | |||
==I2056== | ==I2056== | ||
Latest revision as of 11:49, 6 July 2007
T9002
- Ligated sequential and double digests of T9002 to 3K3
- Transforming in MG1655
E0240
- Re-doing BB PCR with vent and phusion
- Mix for rxn (100uL):
- 8uL 2.5mM dNTP
- 20uL phusion buffer
- 1.5uL f primer
- 1.5uL r primer
- 1uL DNA
- 67uL H2O
- Just realized that we skipped the ligation step completely (3K3 and 1AK3).
- Doing that now, discarding PCR product.
I2056
- Re-culturing from plate
I2055
- Checked the sequences; none of them have the promoter.
- Waiting for primers to insert the promoter.
