Registry/Measurement kit/Notebook/2007-7-30

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 7: Line 7:
*Should pick some promoters to drop in.
*Should pick some promoters to drop in.
*R0040 + a strong promoter + a weak promoter
*R0040 + a strong promoter + a weak promoter
-
*Some possibilities:
+
*Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
-
**<bbpart>R0051</bbpart>
+
-
**<bbpart>I14032</bbpart>
+
-
**<bbpart>J52034</bbpart>
+
-
**<bbpart>J23100</bbpart> - anderson's promoter library
+
==I2055==
==I2055==
*Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)
*Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)

Revision as of 15:32, 30 July 2007

To Do

  • See about making competent DB3.1 cells
  • Test the top10 stock with a + control (pUC18)
  • See what we can do with BB'd E0240 & I2057

Promoter Testers

  • Should pick some promoters to drop in.
  • R0040 + a strong promoter + a weak promoter
  • Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)

I2055

  • Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)
Personal tools