Registry/Measurement kit/Notebook/2007-7-30

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**<bbpart>J52034</bbpart>
**<bbpart>J52034</bbpart>
**<bbpart>J23100</bbpart> - anderson's promoter library
**<bbpart>J23100</bbpart> - anderson's promoter library
 +
 +
==I2055==
 +
*Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)

Revision as of 11:10, 30 July 2007

To Do

  • See about making competent DB3.1 cells
  • Test the top10 stock with a + control (pUC18)
  • See what we can do with BB'd E0240 & I2057

Promoter Testers

  • Should pick some promoters to drop in.
  • R0040 + a strong promoter + a weak promoter
  • Some possibilities:
    • <bbpart>R0051</bbpart>
    • <bbpart>I14032</bbpart>
    • <bbpart>J52034</bbpart>
    • <bbpart>J23100</bbpart> - anderson's promoter library

I2055

  • Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)
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