Registry/Measurement kit/Notebook/2007-7-28: Difference between revisions
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*Gel images: | *Gel images: | ||
[[Image:7-28-lrg.jpg|thumb|left]] | [[Image:7-28-lrg.jpg|thumb|left]] | ||
* | *Large gel: | ||
**I2057-ES (2,1); I2057-EX (6-1) | **I2057-ES (2,1); I2057-EX (6-1) | ||
**E0240-ES(4-1); I2057-ES(6-3) | **E0240-ES(4-1); I2057-ES(6-3) | ||
[[Image:7-28-07-sm.jpg|thumb|left]] | [[Image:7-28-07-sm.jpg|thumb|left]] | ||
*E0240-EX (6-1); E0240-ES(6,5) | *Small gel: | ||
*I2056-1AK3 (2,1); I2057-1AK3 (2,1); E0240-1AK3 (1); I2055-1AK3(3-1) | **E0240-EX (6-1); E0240-ES(6,5) | ||
**I2056-1AK3 (2,1); I2057-1AK3 (2,1); E0240-1AK3 (1); I2055-1AK3(3-1) | |||
Latest revision as of 07:24, 30 July 2007
Overnight cultures
- Growth on:
- E0240-1AK3
- I2055-1AK3
- I2057-1AK3
- I2056-1AO3
- Both promoter testers (3K3, ligated at NotI) versions E-X and E-S
- None:
- F2620-1AK3 and 3K3
- Replate of F2620-1AK3
I2055-1AK3
- Ran gel of last night's PCR, all colonies were wrong size.
Colony PCRs
- Gel images:
- Large gel:
- I2057-ES (2,1); I2057-EX (6-1)
- E0240-ES(4-1); I2057-ES(6-3)
- Small gel:
- E0240-EX (6-1); E0240-ES(6,5)
- I2056-1AK3 (2,1); I2057-1AK3 (2,1); E0240-1AK3 (1); I2055-1AK3(3-1)
- Made overnights of:
- I2057-EX #6
- I2057-ES #4
- E0240-EX #1
- E0240-ES #2
- E0240-1AK3 #1 (not biobricked)
Transformations
- Ligated ccdB into all promoter testers and transformed.
