Registry/Measurement kit/Notebook/2007-7-25: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 11: | Line 11: | ||
*Ligating into 1AK3; ran out of 3K3. | *Ligating into 1AK3; ran out of 3K3. | ||
==Transformations/ | ==Transformations/overnight stuff== | ||
*O/n of 3K3 | *O/n of 3K3 | ||
*Transformation of F2620-3K3 (2 samples), F2620-1AK3, and I2056-1AK3. | *Transformation of F2620-3K3 (2 samples), F2620-1AK3, and I2056-1AK3. | ||
*Digesting 1AK3 E/X, E/P | |||
Revision as of 10:52, 25 July 2007
T9002
- F2620 was ligated into 3K3(E/P) instead of E/X
- Re-ligated F2620 (M/X) into 3K3 (E/X) and 1AK3 (E/X)
- Reaction with 3K3 was left in incubator too long. Made another reaction, will transform both.
Misc
Also Digesting more 3K3 (E/P)Making o/n of 3K3- Mini-prepped 1AK3, digested E/X, E/P
I2056
- Ligating into 1AK3; ran out of 3K3.
Transformations/overnight stuff
- O/n of 3K3
- Transformation of F2620-3K3 (2 samples), F2620-1AK3, and I2056-1AK3.
- Digesting 1AK3 E/X, E/P
