Registry/Measurement kit/Notebook/2007-7-19: Difference between revisions
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==Primer order== | ==Primer order== | ||
TCAGCGAT ATGCAT TATAAACGCAGAAAGGCCCAC, E0240_T9002_rev | *TCAGCGAT ATGCAT TATAAACGCAGAAAGGCCCAC, E0240_T9002_rev | ||
8-merR.NotI.EcoRI.CAGAAATCATCCTTAGCGAAAGC, BB_P_Rev_Eco | *8-merR.NotI.EcoRI.CAGAAATCATCCTTAGCGAAAGC, BB_P_Rev_Eco | ||
8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Spe | *8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Spe | ||
8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Xba | *8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Xba | ||
8-merR.NotI.SpeI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Spe | *8-merR.NotI.SpeI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Spe | ||
8-merR.NotI.EcoRI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Eco | *8-merR.NotI.EcoRI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Eco | ||
8-merF.NotI.XbaI.ATGCGTAAAGGAGAAGAACTTTTC, BB_I2055_Fwd_Xba | *8-merF.NotI.XbaI.ATGCGTAAAGGAGAAGAACTTTTC, BB_I2055_Fwd_Xba | ||
8-merR.NotI.EcoRI.TTTATTCGACTATAACAAACCATTTTC, BB_T9002_Rev_Eco | *8-merR.NotI.EcoRI.TTTATTCGACTATAACAAACCATTTTC, BB_T9002_Rev_Eco | ||
8-merF.NotI.EcoRI.TCACACAGGAAAGTACTAGATGCG, BB_T9002_Fwd_Pst | *8-merF.NotI.EcoRI.TCACACAGGAAAGTACTAGATGCG, BB_T9002_Fwd_Pst | ||
CTTAGTAG.CAATTG.tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag.ATGCGTAAAGGAGAAGAACTTTTC , I2055_promoter_F | *CTTAGTAG.CAATTG.tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag.ATGCGTAAAGGAGAAGAACTTTTC , I2055_promoter_F | ||
*TCAGCGAT.ATGCAT.TATAAACGCAGAAAGGCCCAC, E0240_R | |||
TCAGCGAT.ATGCAT.TATAAACGCAGAAAGGCCCAC, E0240_R | *8-merF.NotI.XbaI.ATGGCTTCCTCCGAAGACG, BB_I2056_Fwd_Xba | ||
8-merF.NotI.XbaI.ATGGCTTCCTCCGAAGACG, BB_I2056_Fwd_Xba | *8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Spe | ||
8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Spe | *8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Xba | ||
8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Xba | |||
Revision as of 00:16, 20 July 2007
Primers
- Creating new E0240 reverse primer for T9002: (NsiI)
- TCAGCGAT ATGCAT TATAAACGCAGAAAGGCCCAC
- Yesterday's PCR didn't work, as expected.
- Made new ladder dilution:
- 1µL ladder
- 9µL water
- 2µL loading buff
Primer order
- TCAGCGAT ATGCAT TATAAACGCAGAAAGGCCCAC, E0240_T9002_rev
- 8-merR.NotI.EcoRI.CAGAAATCATCCTTAGCGAAAGC, BB_P_Rev_Eco
- 8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Spe
- 8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGCG, BB_E0240_Fwd_Xba
- 8-merR.NotI.SpeI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Spe
- 8-merR.NotI.EcoRI.GTGCTCAGTATCTCTATCACTGATAGG, BB_R_Rev_Eco
- 8-merF.NotI.XbaI.ATGCGTAAAGGAGAAGAACTTTTC, BB_I2055_Fwd_Xba
- 8-merR.NotI.EcoRI.TTTATTCGACTATAACAAACCATTTTC, BB_T9002_Rev_Eco
- 8-merF.NotI.EcoRI.TCACACAGGAAAGTACTAGATGCG, BB_T9002_Fwd_Pst
- CTTAGTAG.CAATTG.tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag.ATGCGTAAAGGAGAAGAACTTTTC , I2055_promoter_F
- TCAGCGAT.ATGCAT.TATAAACGCAGAAAGGCCCAC, E0240_R
- 8-merF.NotI.XbaI.ATGGCTTCCTCCGAAGACG, BB_I2056_Fwd_Xba
- 8-merF.NotI.SpeI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Spe
- 8-merF.NotI.XbaI.TCACACAGGAAAGTACTAGATGGC, BB_I2057_Fwd_Xba
