Registry/Measurement kit/Notebook/2007-6-28: Difference between revisions
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*Re-transforming B0032 from iGEM distro | *Re-transforming B0032 from iGEM distro | ||
*Also running digest of B0032 from old prep | *Also running digest of B0032 from old prep | ||
*Plating rest of the culture |
Latest revision as of 11:02, 28 June 2007
To Do
- Decide how to re-do PCR
- Problem is likely dNTP dilution
Device culture
- No growth once again.
PCR
- Re-doing all PCRs, with new dNTP dilution (2.5 mM)
Construction of promoter tester
- Re-transforming B0032 from iGEM distro
- Also running digest of B0032 from old prep
- Plating rest of the culture