Registry/Measurement kit/Notebook/2007-6-14: Difference between revisions
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#Digest 5 constructs | #Digest 5 constructs | ||
#re-Evaluate design based on recommendations from group meeting | #re-Evaluate design based on recommendations from group meeting | ||
==Mini-prep of cultures== | |||
*made glycerol stocks - 1mL of culture in each --> -80 freezer. | |||
*placed remaining culture in 1.5mL tubes, spun at 5k for 4 min | |||
===notes=== | |||
*extra reagents in Kit Reagents. 3rd shelf in 2nd room fridge | |||
*protocol in blue booklet | |||
*get ~150mL beaker for supernatant | |||
*be sure to shake the reagents from the kit a little before using them | |||
*resuspend the whole culture (not individual pellets) in 250uL of P1. |
Revision as of 11:53, 14 June 2007
To Do
- Update Registry/Measurement kit/Glycerol box
- Miniprep 5 constructs
- Digest 5 constructs
- re-Evaluate design based on recommendations from group meeting
Mini-prep of cultures
- made glycerol stocks - 1mL of culture in each --> -80 freezer.
- placed remaining culture in 1.5mL tubes, spun at 5k for 4 min
notes
- extra reagents in Kit Reagents. 3rd shelf in 2nd room fridge
- protocol in blue booklet
- get ~150mL beaker for supernatant
- be sure to shake the reagents from the kit a little before using them
- resuspend the whole culture (not individual pellets) in 250uL of P1.