Registry/Measurement kit/Notebook/2007-6-13

Registry/Measurement_kit/Notebook

Colony PCR

• 1-3: #1 (<bbpart>E0240</bbpart>) -- #3,size OK
• 4-6: #2 (<bbpart>R0040</bbpart>) -- #6,size OK
• 7-9: #3 (<bbpart>I13401</bbpart>) -- #9,size OK (sort of, 7&8 are slightly smaller)
• 10-12: #4 (#12 bad) (<bbpart>J04650</bbpart>) -#11, size OK
• 13: #5 (<bbpart>B0032</bbpart>) -- #13,size OK

• note: reagents in PCR TOOLS box in fridge, Taq is in Shared polymerase in the fridge near entrance

Analytical gel

• lane 1: ladder empty
• lanes 2-13: samples as above
• lane 14: ladder
• lanes 15-18: jason's stuff

Gel from 6/13/07

2 log ladder

Overnight cultures

• inoculated 5mL of LB+5µL of Amp. with 20µL of cell suspension from the colonies denoted in section 1.