Refolding Proteins: Difference between revisions
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Arthur K Yu (talk | contribs) (New page: ==Introduction== Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfo...) |
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Revision as of 10:10, 12 August 2008
Introduction
Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify denatured protein from these inclusion bodies and then refold them manually.
Resources
- Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding
- Sauer:Purification_of_His-tagged_proteins/Denaturing_prep
- REFOLD - Repository of refolding protocols for specific protocols.
See also
- IGEM:IMPERIAL/2007/CFS - Cell free systems for protein expression.
