Recombineering
Recombineering is a method where E-coli undergoes recombination between linear DNA, introduced through electroporation, with circularized DNA already present within the cell.
http://recombineering.ncifcrf.gov/
[math]\displaystyle{ \lambda }[/math] phage exo, bet, and gam genes are integrated into E-coli to demonstrate phage promoted homologous recombination.
Murphy 1998 [math]\displaystyle{ \lambda }[/math] Red-Promoted Gene Replacement
To acheive control over phage recombination gene activity, temperature sensitive [math]\displaystyle{ \lambda }[/math] repressor (cI857) was introduced.
Yu 2000 An efficient recombination system for chromosome engineering in Escherichia coli
This process is then adapted to E-coli strain DH10B, a Bacteria Artificial Chromosome (BAC)strain. This results in the original recombineering E-coli strain designated DY380 as well as other versions modified to include different selection mechanisms.
Lee 2001. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA
All about the [math]\displaystyle{ \lambda }[/math] phage recombination proteins, exonuclease, [math]\displaystyle{ \beta }[/math], and [math]\displaystyle{ \gamma }[/math]!!
An Exonuclease Induced by phage [math]\displaystyle{ \lambda }[/math]. Little 1967
Roles of Exonuclease and [math]\displaystyle{ \beta }[/math] protein of phage [math]\displaystyle{ \lambda }[/math] in recombination. Carter 1971
[math]\displaystyle{ \beta }[/math] protein of phage [math]\displaystyle{ \lambda }[/math] promotes renaturation of DNA. Kmiec 1981
Pairing activities of [math]\displaystyle{ \beta }[/math] protein in phage [math]\displaystyle{ \lambda }[/math]. Muniyappa 1986
How [math]\displaystyle{ \gamma }[/math] protein specified by phage [math]\displaystyle{ \lambda }[/math] affects recBC enzyme in E. Coli. Karu 1975
[math]\displaystyle{ \gamma }[/math] protein of phage [math]\displaystyle{ \lambda }[/math] inhibits recombination activites of RecBCD enzyme. Murphy 1991