Recombineering: Difference between revisions
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[http://www.pnas.org/cgi/content/abstract/97/11/5978 Yu 2000] An efficient recombination system for chromosome engineering in Escherichia coli | [http://www.pnas.org/cgi/content/abstract/97/11/5978 Yu 2000] An efficient recombination system for chromosome engineering in Escherichia coli | ||
This process is then adapted to E-coli strain DH10B, a Bacteria Artificial Chromosome (BAC)strain. This results in the original recombineering E-coli strain designated DY380 as well as other selection modified versions. | |||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11352566 Lee 2001]. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. | |||
Revision as of 15:51, 27 April 2006
Recombineering is a method where E-coli undergoes recombination between linear DNA, introduced through electroporation, with circularized DNA already present within the cell.
http://recombineering.ncifcrf.gov/
Lambda phage exo, bet, gam gene integration into E-coli to demonstrate phage promoted homologous recombination. Murphy 1998 [math]\displaystyle{ lambda }[/math] Red-Promoted Gene Replacement
To acheive control over phage recombination genes, temperature sensitive lambda repressor (cI857) was introduced. Yu 2000 An efficient recombination system for chromosome engineering in Escherichia coli
This process is then adapted to E-coli strain DH10B, a Bacteria Artificial Chromosome (BAC)strain. This results in the original recombineering E-coli strain designated DY380 as well as other selection modified versions. Lee 2001. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.
