Reagents for SPR: Difference between revisions
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aliquote into 200μL portions directly into vials to be used on the instrument<br> | aliquote into 200μL portions directly into vials to be used on the instrument<br> | ||
freeze the aliquotes and store at -20 for no more then 2 months<br> | freeze the aliquotes and store at -20 for no more then 2 months<br> | ||
if you buy EDC from Biacore - | if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 9.781 mL water | ||
|- | |- | ||
|N-hydroxysuccinimide ('''NHS''')<br> | |N-hydroxysuccinimide ('''NHS''')<br> | ||
| Line 18: | Line 18: | ||
|115.09 | |115.09 | ||
|NHS stock solution 0.1M<br> | |NHS stock solution 0.1M<br> | ||
prepare and store as desribed for EDC | prepare and store as desribed for EDC<br> | ||
Biacore supplied 115 mg portion should be dissolved in 10 mL water | |||
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[[Category:SPR]] | [[Category:SPR]] | ||
Revision as of 14:05, 19 May 2008
| reagents | purpose | Mr | handling instructions |
| 1-ethyl-3-(3-dimethyl
aminopropyl) carbodiimide (EDC) |
amine coupling | 191.70 | EDC stock solution 0.4M resuspend fresh ultrapure reagent in milli-Q water |
| N-hydroxysuccinimide (NHS) |
amine coupling | 115.09 | NHS stock solution 0.1M prepare and store as desribed for EDC |
