RNA extraction using trizol/tri

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RNA extraction with TRIZOL (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total [[RNA extraction]] from cells. It takes slightly longer than column-based methods like RNAeasy but it has higher capacity and can yield more RNA.
== Principle ==
== Principle ==
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* [http://www.animal.ufl.edu/hansen/protocols/RNA_extraction.htm RNA extraction with TRIZOL] (Uni Florida)
* [http://www.animal.ufl.edu/hansen/protocols/RNA_extraction.htm RNA extraction with TRIZOL] (Uni Florida)
* [http://www.utm.utoronto.ca/~w3flyma/protocols/Trizol%20Troubleshooting.pdf Troubleshooting guide for TRIZOL extraction] (Uni Toronto)
* [http://www.utm.utoronto.ca/~w3flyma/protocols/Trizol%20Troubleshooting.pdf Troubleshooting guide for TRIZOL extraction] (Uni Toronto)
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Tips
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* [http://www.ambion.com/techlib/tn/94/949.html Ambion tips on RNA extraction]

Revision as of 14:07, 14 February 2008

RNA extraction with TRIZOL (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extraction from cells. It takes slightly longer than column-based methods like RNAeasy but it has higher capacity and can yield more RNA.

Contents

Principle

Reagents

Steps

Common mistakes

See also

External links

Reagents

Protocols

Tips

Personal tools