RNA extraction: Difference between revisions
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This is a general protocol page for extracting RNA from samples. | |||
==Specific Protocols== | ==Specific Protocols== | ||
*[[Endy:RNA extraction]] | *[[Endy:RNA extraction]] | ||
| Line 8: | Line 8: | ||
==Notes== | ==Notes== | ||
Some general notes on isolating RNA. | |||
These notes are focused on bacterial cells but please contribute to include information about plant, mammalian and other cells etc. | |||
#Centrifugation of cells can induce a stress response in cells altering transcript levels (potentially unequally across different transcripts). | |||
#[http://www1.qiagen.com/literature/handbooks/literature.aspx?id=1000278 "RNA''protect''"] is a reagent used to stabilize RNA content in bacterial cells grown in liquid culture. | |||
#*Works best if cells are grown in minimal media. Works worst in LB liquid culture due to complex components. | |||
==Related information== | |||
See [[RNA]]. | |||
==Reference== | |||
<biblio> | |||
#MeasuringGeneExpression isbn=0415374723 | |||
</biblio> | |||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:RNA]] | [[Category:RNA]] | ||
[[Category:In vitro]] | [[Category:In vitro]] | ||
Revision as of 09:34, 9 October 2007
This is a general protocol page for extracting RNA from samples.
Specific Protocols
- Endy:RNA extraction
- Extraction of RNA from tissues in English
- Extraction of RNA from tissues in French
- Gattuso:RNA extraction
Notes
Some general notes on isolating RNA.
These notes are focused on bacterial cells but please contribute to include information about plant, mammalian and other cells etc.
- Centrifugation of cells can induce a stress response in cells altering transcript levels (potentially unequally across different transcripts).
- "RNAprotect" is a reagent used to stabilize RNA content in bacterial cells grown in liquid culture.
- Works best if cells are grown in minimal media. Works worst in LB liquid culture due to complex components.
Related information
See RNA.
